Seybold W D, Meltzer P S, Mitchell H K
Biochem Genet. 1975 Feb;13(1-2):85-108. doi: 10.1007/BF00486009.
Our earlier evidence concerning the complexity of the activation process for Drosophila phenol oxidase has been confirmed by separation and purification of six proteins involved. This is a minimal number required in a reaction series or cascade to yield active enzyme, and at least two more proteins are known to be involved. A simpler system involving only the last step with one precursor and one activation as has been reported in the literature is consistent with the cascade picture, but the whole complex system must be considered when dealing with genetic and developmental regulation of pigmentation and sclerotization. Details are given for partial or complete purification of six of the proteins involved and evidence for their modes of interaction is presented.
我们早期关于果蝇酚氧化酶激活过程复杂性的证据，已通过对所涉及的六种蛋白质的分离和纯化得到证实。这是反应系列或级联反应中产生活性酶所需的最少蛋白质数量，并且已知至少还有另外两种蛋白质参与其中。文献中报道的一个仅涉及最后一步、有一个前体和一次激活的更简单系统，与级联反应的情况相符，但在处理色素沉着和硬化的遗传及发育调控时，必须考虑整个复杂系统。文中给出了所涉及的六种蛋白质的部分或完全纯化的详细信息，并展示了它们相互作用模式的证据。
