铁缺乏诱导HL-60细胞凋亡。
Iron deprivation-induced apoptosis in HL-60 cells.
作者信息
Fukuchi K, Tomoyasu S, Tsuruoka N, Gomi K
机构信息
Department of Clinical Pathology, School of Medicine, Showa University, Tokyo, Japan.
出版信息
FEBS Lett. 1994 Aug 15;350(1):139-42. doi: 10.1016/0014-5793(94)00755-1.
Iron deprivation of HL-60 cells with deferoxamine B mesylate (DFO) induced apoptosis. DNA fragmentation became apparent with 10(-6) M DFO after 48 h treatment. The apoptosis peak according to the DNA histogram on flow cytometry and typical nuclear collapse and were observed microscopically after 48 h treatment with 10(-4) M DFO. Cells treated with 10(-4) M DFO for as little as 24 h were shown to be committed to apoptosis, as chromatin condensation progressed gradually thereafter.
用甲磺酸去铁胺（DFO）剥夺HL-60细胞的铁会诱导细胞凋亡。在10⁻⁶M DFO处理48小时后，DNA片段化变得明显。在用10⁻⁴M DFO处理48小时后，通过流式细胞仪的DNA直方图观察到凋亡峰以及典型的核固缩，并在显微镜下观察到。用10⁻⁴M DFO处理仅24小时的细胞就显示出会发生凋亡，因为此后染色质凝聚逐渐进展。
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