多胺在表皮生长因子（EGF）、转化生长因子（TGF）-α和-β1对L6及胎牛成肌细胞培养作用中的参与情况。

Involvement of polyamines in epidermal growth factor (EGF), transforming growth factor (TGF)-alpha and -beta 1 action on culture of L6 and fetal bovine myoblasts.

Blachowski S, Motyl T, Grzelkowska K, Kasterka M, Orzechowski A, Interewicz B

Department of Animal Physiology, Veterinary Faculty, Warsaw Agricultural University, Poland.

Int J Biochem. 1994 Jul;26(7):891-7. doi: 10.1016/0020-711x(94)90082-5.

Abstract

alpha-Difluoromethylornithine, an irreversible inhibitor of ornithine decarboxylase significantly abolished stimulation of protein synthesis evoked by EGF, TGF-alpha or -beta 1 in L6 and fetal bovine myoblasts. 2. The participation of polyamines in early events evoked by growth factors was shown by a significant stimulation of ornithine decarboxylase and S-adenosylmethionine decarboxylase activity as well as increased concentration of spermidine and spermine in L6 cells exposed to TGF-alpha and EGF. 3. TGF-beta 1 at a high concentration (1 ng/ml) increased protein synthesis in L6 myoblasts but inhibited it in fetal bovine myoblasts. Metabolic effects of TGF-beta 1 in L6 cells was associated with an enhancement of decarboxylase activities, however there were no significant changes in cellular polyamine concentrations. Presented data suggest that polyamines are involved in the signal transduction pathway of EGF, TGF-alpha, and -beta 1 in L6 and fetal bovine myoblasts.

摘要

α-二氟甲基鸟氨酸是鸟氨酸脱羧酶的不可逆抑制剂，它能显著消除表皮生长因子（EGF）、转化生长因子-α（TGF-α）或转化生长因子-β1（TGF-β1）在L6和胎牛成肌细胞中所引起的蛋白质合成刺激。2. 多胺参与生长因子引发的早期事件，这表现为在暴露于TGF-α和EGF的L6细胞中，鸟氨酸脱羧酶和S-腺苷甲硫氨酸脱羧酶活性显著增强，以及亚精胺和精胺浓度升高。3. 高浓度（1 ng/ml）的TGF-β1可增加L6成肌细胞中的蛋白质合成，但在胎牛成肌细胞中则抑制蛋白质合成。TGF-β1在L6细胞中的代谢效应与脱羧酶活性增强有关，然而细胞内多胺浓度并无显著变化。现有数据表明，多胺参与了L6和胎牛成肌细胞中EGF、TGF-α和TGF-β1的信号转导途径。