Dawirs R R, Teuchert-Noodt G, Czaniera R
Department of Neuroanatomy, Faculty of Biology, University of Bielefeld, Germany.
J Hirnforsch. 1994;35(2):195-204.
Dopamine (DA)-immunoreactivity was investigated in the prefrontal cortex (PFC) of 90 day old adult male gerbils (Meriones unguiculatus) after they had received a single dose of either methamphetamine (50 mg/kg; i.p.) or saline at the age of postnatal day 14. For that purpose, a selective and sensitive antibody directed against glutaraldehyde-conjugated dopamine was applied. All detectable fragments of dopamine-immunoreactive fibres were identified in consecutive frontal sections of the pregenual prefrontal cortex, and their total numbers and total length were determined in the medial (mPFC) and orbital prefrontal cortex (oPFC). The results indicate that a single application of methamphetamine during early postnatal development caused a significant and severe restraint of the subsequent maturation of the prefrontal dopamine-innervation. Although, on postnatal day 14, the total dopamine-immunoreactivity had only attained about 4% (mPFC) and 7% (oPFC) of the regular adult values, this solitary pharmacological challenge entailed final adult innervation densities which were about 38% (mPFC) and 50% (oPFC) below those of the controls. Considering the pivotal role which mesoprefrontal dopaminergic afferents play in morphogenesis and regular functioning, the present results are discussed with current understanding of structural and functional plasticity during maturation of the prefrontal cortex.
在出生后第14天给90日龄成年雄性沙鼠（长爪沙鼠）单次注射甲基苯丙胺（50毫克/千克；腹腔注射）或生理盐水后，研究其前额叶皮质（PFC）中的多巴胺（DA）免疫反应性。为此，应用了一种针对戊二醛结合多巴胺的选择性和敏感性抗体。在膝前区前额叶皮质的连续额叶切片中识别出所有可检测到的多巴胺免疫反应性纤维片段，并在内侧前额叶皮质（mPFC）和眶前额叶皮质（oPFC）中确定其总数和总长度。结果表明，在出生后早期发育期间单次应用甲基苯丙胺会显著且严重地抑制前额叶多巴胺神经支配的后续成熟。虽然在出生后第14天，多巴胺免疫反应性总量仅达到正常成年值的约4%（mPFC）和7%（oPFC），但这种单一的药理学刺激导致最终成年神经支配密度比对照组低约38%（mPFC）和50%（oPFC）。考虑到中前额叶多巴胺能传入神经在形态发生和正常功能中所起的关键作用，结合当前对前额叶皮质成熟过程中结构和功能可塑性的理解对本研究结果进行了讨论。
