Pampfer S, Moulaert B, Vanderheyden I, Wuu Y D, De Hertogh R
Physiology of Human Reproduction Research Unit, University of Louvain, School of Medicine, Brussels, Belgium.
J Reprod Fertil. 1994 May;101(1):199-206. doi: 10.1530/jrf.0.1010199.
Tumour necrosis factor alpha (TNF-alpha) synthesis has recently been described in the uterus during the preimplantation phase of pregnancy. The present study was undertaken to determine whether preimplantation embryos are a potential target for TNF-alpha in rats. First, the expression of TNF-alpha receptors by blastocysts was demonstrated by ligand binding assay with human 125I-labelled TNF-alpha and reverse transcription-amplification for the p60 receptor form. The functionality of these receptors was then assessed by incubating blastocysts with 3 nmol mouse TNF-alpha l-1 in vitro and determining their morphology and the number of cells after 24 h. At that concentration, cell proliferation in blastocysts was inhibited by TNF-alpha but this was not accompanied by any change in the morphology of the embryos. Similar results were obtained when lower doses of TNF-alpha (30 and 300 pmol l-1) were tested. The rate of glucose consumption of rat blastocysts exposed to 3 nmol TNF-alpha l-1 was not altered when they were incubated with the cytokine for 4 h, but the rate of glucose incorporation decreased over the same period. Our data indicate that rat blastocysts are responsive to physiological concentration of TNF-alpha and that this cytokine has the potential to influence the preimplantation development of rats.
肿瘤坏死因子α（TNF-α）的合成最近在妊娠植入前阶段的子宫中已有报道。本研究旨在确定植入前胚胎是否是大鼠体内TNF-α的潜在作用靶点。首先，通过用人125I标记的TNF-α进行配体结合测定以及对p60受体形式进行逆转录扩增，证明了囊胚中TNF-α受体的表达。然后通过在体外将囊胚与3 nmol小鼠TNF-α l-1孵育，并在24小时后确定其形态和细胞数量，来评估这些受体的功能。在该浓度下，TNF-α抑制了囊胚中的细胞增殖，但这并未伴随胚胎形态的任何变化。当测试较低剂量的TNF-α（30和300 pmol l-1）时，也获得了类似的结果。暴露于3 nmol TNF-α l-1的大鼠囊胚在与细胞因子孵育4小时时，其葡萄糖消耗率没有改变，但在同一时期葡萄糖掺入率下降。我们的数据表明，大鼠囊胚对生理浓度的TNF-α有反应，并且这种细胞因子有可能影响大鼠的植入前发育。
