Yoshimura Y, Hosoi Y, Atlas S J, Ghodgaonkar R, Dubin N H, Dharmarajan A M, Wallach E E
Department of Gynecology and Obstetrics, Johns Hopkins University School of Medicine, Baltimore, MD 21287.
J Reprod Fertil. 1994 May;101(1):207-12. doi: 10.1530/jrf.0.1010207.
The role of cAMP in ovulation, oocyte maturation and prostaglandin production was assessed using a rabbit ovary preparation perfused in vitro. Dibutyryl cAMP (10(-3), 10(-4) or 10(-5) mol l-1) was added to the perfusate of one ovary. The contralateral, control ovary was perfused with medium alone. Thirty minutes after the onset of perfusion, 50 iu hCG was added to the perfusate of all ovaries. Dibutyryl cAMP inhibited hCG-induced ovulation in a dose-related fashion. No difference in ovum maturity or degeneration was found between control ovaries and ovaries treated with dibutyryl cAMP. Ovarian progesterone production was not affected by exposure to dibutyryl cAMP. The concentrations of 6-keto PGF1 alpha (the stable metabolite of prostacyclin) and PGF2 alpha in the perfusate of ovaries treated with dibutyryl cAMP were 49.6% and 32.0% of the control values, respectively, 12 h after hCG administration. Inhibition of 6-keto PGF1 alpha production by dibutyryl cAMP was dose related. Production of PGE2 was unaffected by dibutyryl cAMP. These data raise the possibility that continuous exposure to dibutyryl cAMP may inhibit hCG-induced ovulation in the perfused rabbit ovary via a reduction in PGF2 alpha and prostacyclin production.
使用体外灌注的兔卵巢制剂评估了环磷酸腺苷（cAMP）在排卵、卵母细胞成熟和前列腺素产生中的作用。将二丁酰环磷酸腺苷（10⁻³、10⁻⁴或10⁻⁵mol l⁻¹）添加到一侧卵巢的灌注液中。对侧对照卵巢仅用培养基灌注。灌注开始30分钟后，向所有卵巢的灌注液中添加50国际单位的人绒毛膜促性腺激素（hCG）。二丁酰环磷酸腺苷以剂量相关的方式抑制hCG诱导的排卵。对照卵巢与用二丁酰环磷酸腺苷处理的卵巢之间在卵子成熟或退化方面未发现差异。暴露于二丁酰环磷酸腺苷对卵巢孕酮的产生没有影响。在给予hCG 12小时后，用二丁酰环磷酸腺苷处理的卵巢灌注液中6-酮-前列腺素F1α（前列环素的稳定代谢产物）和前列腺素F2α的浓度分别为对照值的49.6%和32.0%。二丁酰环磷酸腺苷对6-酮-前列腺素F1α产生的抑制作用与剂量相关。二丁酰环磷酸腺苷对前列腺素E2的产生没有影响。这些数据增加了一种可能性，即持续暴露于二丁酰环磷酸腺苷可能通过降低前列腺素F2α和前列环素的产生来抑制灌注兔卵巢中hCG诱导的排卵。
