Van Reeth C, Le Moel G, Lasne Y, Revenant M C, Agneray J, Kahn M F, Bourgeois P
Rheumatology Department, Pitié Hospital, Paris, France.
J Rheumatol. 1994 May;21(5):890-5.
Still's disease is an acute systemic inflammatory disorder. There are no pathognomonic symptoms or specific laboratory abnormalities. Serum ferritin concentration in rheumatoid arthritis together with some plasma glycoproteins such as alpha 2-glycoprotein and C-reactive protein are part of the response to inflammation. Ferritin in plasma is glycosylated and the sialoglycosylated forms increase its microheterogeneity. Our purpose was to confirm in a large series that high values of ferritin can be found in adult Still's disease (ASD) and to see if a specific isoferritin can be isolated in this disease compared with the other systemic diseases.
Thirty-one sera were investigated from 11 men and 9 women with ASD and compared with 27 sera from 27 patients with systemic diseases. We studied the course of one case of ASD for 15 months. Serum ferritin was determined by immunoenzymology (Abbott Ferrizin). The isoferritins were investigated by isoelectric focussing and the percentage of glycosylation by affinity for concanavalin A (Con-A).
In patients with active ASD, the ferritin levels were higher than in patients with inactive ASD or other systemic diseases: p < 0.001. The glycoforms of ferritin were basic and the proportion of ferritin bound to Con-A was lower than other ASD: p < 0.001.
Serum ferritin levels have a diagnostic value for acute ASD. The study of sialylation and abnormalities in the glycosylation of ferritin helps to discriminate ASD from arthritis or other systemic diseases. In conclusion, the glycoform of isoferritins and the percentage of glycosylation offers an additional tool for the diagnosis of Still's disease.
斯蒂尔病是一种急性全身性炎症性疾病。该病没有特征性症状或特定的实验室异常表现。类风湿关节炎患者的血清铁蛋白浓度以及一些血浆糖蛋白，如α2-糖蛋白和C反应蛋白，是炎症反应的一部分。血浆中的铁蛋白是糖基化的，唾液酸化形式增加了其微观异质性。我们的目的是在大量病例中证实成人斯蒂尔病（ASD）患者可出现高铁蛋白值，并观察与其他全身性疾病相比，该病是否能分离出特定的同工铁蛋白。
对11名男性和9名女性ASD患者的31份血清进行研究，并与27名全身性疾病患者的27份血清进行比较。我们对1例ASD患者进行了15个月的病程研究。采用免疫酶法（雅培Ferrizin）测定血清铁蛋白。通过等电聚焦研究同工铁蛋白，通过伴刀豆球蛋白A（Con-A）亲和力研究糖基化百分比。
活动期ASD患者的铁蛋白水平高于非活动期ASD患者或其他全身性疾病患者：p < 0.001。铁蛋白的糖型为碱性，与Con-A结合的铁蛋白比例低于其他ASD患者：p < 0.001。
血清铁蛋白水平对急性ASD具有诊断价值。铁蛋白唾液酸化和糖基化异常的研究有助于将ASD与关节炎或其他全身性疾病区分开来。总之，同工铁蛋白的糖型和糖基化百分比为斯蒂尔病的诊断提供了额外的工具。
