在大肠杆菌中表达的人血小板肌动蛋白结合蛋白的纯化、表征及结晶
Purification, characterization and crystallization of human platelet profilin expressed in Escherichia coli.
作者信息
Fedorov A A, Pollard T D, Almo S C
机构信息
Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461.
出版信息
J Mol Biol. 1994 Aug 19;241(3):480-2. doi: 10.1006/jmbi.1994.1522.
Human platelet profilin was expressed in Escherichia coli using a T7 based expression vector. The recombinant material is similar to authentic human platelet profilin based on the measured Kd for rabbit skeletal muscle actin. Crystals of the recombinant material were obtained from both PEG 8000 and (NH4)2SO4. These crystals are isomorphous and belong to the monoclinic space group C2, a = 75.0, b = 32.0, c = 62.5, beta = 123 degrees. These crystals contain one molecule in the asymmetric unit and diffract to at least 2.0 A.
使用基于T7的表达载体在大肠杆菌中表达人血小板肌动蛋白结合蛋白。根据所测得的兔骨骼肌肌动蛋白的解离常数（Kd），重组蛋白与天然人血小板肌动蛋白结合蛋白相似。重组蛋白的晶体是从聚乙二醇8000（PEG 8000）和硫酸铵中获得的。这些晶体是同晶型的，属于单斜空间群C2，a = 75.0，b = 32.0，c = 62.5，β = 123°。这些晶体在不对称单元中含有一个分子，并且衍射能力至少达到2.0埃。
Zotero 插件