Fyrberg C, Ryan L, Kenton M, Fyrberg E
Department of Biology, Johns Hopkins University, Baltimore, MD 21218.
J Mol Biol. 1994 Aug 19;241(3):498-503. doi: 10.1006/jmbi.1994.1526.
Recently, several laboratories have described proteins of yeasts, mammals and Drosophila melanogaster that are 35 to 55% identical to conventional actins, but, as yet, little is known about their functions. We have initiated a systematic study by using degenerate oligonucleotides specifying two highly conserved nucleotide-binding peptides of actin, in conjunction with polymerase chain reaction techniques, to isolate Drosophila genes that encode actin-related proteins. Here we summarize the isolation of four such genes and compare the sequences of the proteins that they encode. Computer searches of databases revealed that three of the encoded proteins are homologs of yeast or mammalian actin-related proteins, implying that the corresponding proteins participate in functions common to many cell types. The fourth gene encodes a novel protein that, apparently is expressed within testes. The four genes are located within the 14D, 53D, 66B and 87C subdivisions of polytene chromosomes.
最近，几个实验室描述了酵母、哺乳动物和果蝇中与传统肌动蛋白有35%至55%同源性的蛋白质，但目前对其功能了解甚少。我们通过使用简并寡核苷酸指定肌动蛋白的两个高度保守的核苷酸结合肽，并结合聚合酶链反应技术，启动了一项系统研究，以分离果蝇中编码肌动蛋白相关蛋白的基因。在此，我们总结了四个此类基因的分离情况，并比较了它们所编码蛋白质的序列。数据库的计算机搜索显示，其中三个编码蛋白是酵母或哺乳动物肌动蛋白相关蛋白的同源物，这意味着相应的蛋白质参与了许多细胞类型共有的功能。第四个基因编码一种新蛋白，显然在睾丸中表达。这四个基因位于多线染色体的14D、53D、66B和87C亚区。
