Bridi L C, Sharakhova M V, Sharakhov I V, Cordeiro J, Azevedo Junior G M, Tadei W P, Rafael M S
Programa de Pós-Graduação em Genética, Conservação e Biologia Evolutiva (PP-G GCBEv), Instituto Nacional de Pesquisas da Amazônia (INPA), Manaus, AM, Brazil.
Med Vet Entomol. 2013 Mar;27(1):118-21. doi: 10.1111/j.1365-2915.2012.01019.x. Epub 2012 Jul 16.
Physical and genetic maps have been used for chromosomal localization of genes in vectors of infectious diseases. The availability of polytene chromosomes in malaria mosquitoes provides a unique opportunity to precisely map genes of interest. We report the physical mapping of two actin genes on polytene chromosomes of the major malaria vector in the Amazon, Anopheles darlingi (Diptera: Culicidae). Clones with actin gene sequences were obtained from a cDNA library constructed from RNA isolated from adult females and males of An. darlingi. Each of the two clones was mapped to a unique site on chromosomal arm 2L in subdivisions 21A (clone pl05-A04) and 23B (clone pl17-G06). The obtained results, together with previous mapping data, provide a suitable basis for comparative genomics and for establishing chromosomal homologies among major malaria vectors.
物理图谱和遗传图谱已被用于传染病载体中基因的染色体定位。疟蚊多线染色体的存在为精确绘制感兴趣的基因图谱提供了独特的机会。我们报告了在亚马逊地区主要疟疾传播媒介达林按蚊（双翅目：蚊科）的多线染色体上对两个肌动蛋白基因的物理图谱绘制。含有肌动蛋白基因序列的克隆是从一个cDNA文库中获得的，该文库由从达林按蚊成年雌蚊和雄蚊分离的RNA构建而成。这两个克隆中的每一个都被定位到染色体臂2L上的一个独特位点，分别在21A亚区（克隆pl05 - A04）和23B亚区（克隆pl17 - G06）。所获得的结果，连同先前的图谱数据，为比较基因组学以及在主要疟疾传播媒介之间建立染色体同源性提供了合适的基础。
