Nassif N T, Glenn W K, Mackinlay A G
School of Biochemistry and Molecular Genetics, University of New South Wales, Kensington, Australia.
J Mol Evol. 1994 Jul;39(1):47-55. doi: 10.1007/BF00178248.
Three pairs of oligonucleotide primers based on partial DNA and amino acid sequences were used in a combination of PCR experiments to amplify the beta-globin gene of the bivalve mollusc Anadara trapezia. The sequence of 2,139 bp presented contains the whole of the beta-globin gene with the exception of the 5' flanking sequence. This gene possesses the three-exon-and-two-intron gene structure typical of vertebrate globin genes but the lengths of the introns (762 bp and 690 bp, respectively) are only approximately half the size of those present in a beta-variant gene previously characterized from this organism. The encoded amino acid sequence shows two changes when compared to the previously published amino acid sequence.
基于部分DNA和氨基酸序列设计了三对寡核苷酸引物，用于组合PCR实验，以扩增双壳贝类四角蛤蜊（Anadara trapezia）的β-珠蛋白基因。呈现的2139 bp序列包含整个β-珠蛋白基因，但不包括5'侧翼序列。该基因具有脊椎动物珠蛋白基因典型的三外显子和两内含子基因结构，但内含子长度（分别为762 bp和690 bp）仅约为先前从该生物中鉴定出的β-变体基因中内含子长度的一半。与先前发表的氨基酸序列相比，编码的氨基酸序列有两处变化。
