双壳贝类软体动物梯形不等蛤编码α-和β-珠蛋白链的部分mRNA序列的PCR扩增：α-链C末端氨基酸序列的校正
PCR amplification of partial mRNA sequences encoding the alpha- and beta-globin chains of the bivalve mollusc Anadara trapezia: correction of the C-terminal amino acid sequence of the alpha-chain.
作者信息
Nassif N T, Mackinlay A G, Thompson E O
机构信息
School of Biochemistry and Molecular Genetics, University of New South Wales, Kensington, Australia.
出版信息
Comp Biochem Physiol B. 1993 Jun;105(2):283-7. doi: 10.1016/0305-0491(93)90230-3.
PMID:8359016
Abstract
- PCR was carried out on cDNA which had been synthesized from poly(A)+ RNA extracted from A. trapezia red blood cells. 2. A 348 bp product encoding the C-terminal region of the alpha-globin chain and another product encoding a portion of the beta-globin chain were isolated. 3. The C-terminal alpha-chain sequence, encoded by the cDNA, differs from the previously published sequence and these changes improve the alignment of this chain with the other globin chains of this organism.
摘要
- 对从梯形艾氏鱼红细胞中提取的聚腺苷酸加尾RNA合成的互补DNA（cDNA）进行聚合酶链反应（PCR）。2. 分离出一个编码α-珠蛋白链C端区域的348碱基对产物以及另一个编码β-珠蛋白链一部分的产物。3. 由该cDNA编码的C端α链序列与先前发表的序列不同，这些变化改善了该链与该生物体其他珠蛋白链的比对。
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