通过免疫电子显微镜观察大鼠肺中白细胞毒素（9:10-环氧-12-十八碳烯酸）的细胞间定位。

Intercellular localization of leukotoxin (9:10-epoxy-12-octadecenoic acid) in lungs in rats by an immunoelectron microscopy.

作者信息

Zhang W D, Iwasaka S, Nagao M, Yamada Y, Kobayashi M, Iwadate K, Oono T, Takatori T

机构信息

Department of Forensic Medicine, Faculty of Medicine, University of Tokyo, Japan.

出版信息

Nihon Hoigaku Zasshi. 1994 Jun;48(3):161-5.

PMID:8065066

Abstract

In this paper intercellular localization of leukotoxin (9:10-epoxy-12-octadecenoic acid, LTx) in neutrophils and alveolar macrophages of rats exposed to the 100% oxygen was demonstrated by a pre-embedding method of an immunoelectron microscopy. The Sprague-Dawley rats were treated with the 100% oxygen for 72 hrs, and the lungs were stained with anti-LTx IgG (Fab) by an immunocytochemical method and examined by a transmission electron microscope. The positive reactions with anti-LTx IgG (Fab) was shown strongly in the rough-surfaced endoplasmic reticulum (rER) and cell membranes of the neutrophils and alveolar macrophages. This result indicates that LTx is produced in biomembrane-rich organelle of alveolar macrophages as well as neutrophils dependently on the treatment period under hyperoxia conditions, suggesting that LTx is closely related to biological reactions and is an important chemical mediator on pulmonary diseases.

摘要

本文采用免疫电子显微镜包埋前法，证实了暴露于100%氧气的大鼠中性粒细胞和肺泡巨噬细胞中白细胞毒素（9:10-环氧-12-十八碳烯酸，LTx）的细胞内定位。将Sprague-Dawley大鼠用100%氧气处理72小时，通过免疫细胞化学方法用抗LTx IgG（Fab）对肺进行染色，并用透射电子显微镜检查。抗LTx IgG（Fab）在中性粒细胞和肺泡巨噬细胞的糙面内质网（rER）和细胞膜中显示出强烈的阳性反应。该结果表明，在高氧条件下，LTx在肺泡巨噬细胞以及中性粒细胞富含生物膜的细胞器中产生，这取决于处理时间，提示LTx与生物反应密切相关，是肺部疾病的重要化学介质。