Zhang W, Nagao M, Takatori T, Iwadate K, Itakura Y, Yamada Y, Iwase H, Oono T
Department of Forensic Medicine, Faculty of Medicine, University of Tokyo, Japan.
Int J Legal Med. 1995;107(4):174-8. doi: 10.1007/BF01428400.
This paper investigates the immunohistochemical dynamics of leukotoxin (9,10-epoxy-12-octadecenoic acid, LTx) in the lungs of rats exposed to hyperoxia with or without paraquat. The rats were treated with 100% oxygen or ambient air for 24, 48, 72 and 96 h in the presence or absence of a low or high dose paraquat (1,1'-dimethyl-4,4'-bipyridinium, PQ) injection. Immunostaining for LTx demonstrated positive reactions in the neutrophils that showed a progressive increase in intensity of staining with time in all groups exposed to 100% oxygen and in the group with high dose PQ, but the positive findings were weak in the group injected with low dose PQ only. We found the positive immunostaining reaction not only in neutrophils but also in alveolar macrophages. This indicates that LTx is produced by alveolar macrophages as well as by neutrophils depending on the treatment period under hyperoxic conditions, suggesting that LTx is an important chemical mediator in pulmonary diseases.
本文研究了在有或没有百草枯的情况下，暴露于高氧环境的大鼠肺中白细胞毒素（9,10-环氧-12-十八碳烯酸，LTx）的免疫组化动态变化。在有或没有注射低剂量或高剂量百草枯（1,1'-二甲基-4,4'-联吡啶，PQ）的情况下，将大鼠置于100%氧气或环境空气中处理24、48、72和96小时。对LTx的免疫染色显示，在所有暴露于100%氧气的组以及高剂量PQ组中，中性粒细胞出现阳性反应，且染色强度随时间逐渐增加，但仅注射低剂量PQ的组阳性结果较弱。我们发现不仅在中性粒细胞中，而且在肺泡巨噬细胞中也有阳性免疫染色反应。这表明在高氧条件下，根据处理时间的不同，肺泡巨噬细胞以及中性粒细胞均可产生LTx，提示LTx是肺部疾病中的一种重要化学介质。
