对 Dahl-Iwai 盐敏感大鼠（S）和盐抵抗大鼠（R）的DNA指纹分析。

DNA fingerprint analysis of Dahl-Iwai salt-sensitive rats (S) and salt-resistant rats (R).

作者信息

Yamaguchi S, Umemura S, Iwamoto T, Kobayashi S, Tamura K, Takagi N, Ishii M

机构信息

Second Department of Internal Medicine, Yokohama City University School of Medicine, Japan.

出版信息

Life Sci. 1994;55(9):701-5. doi: 10.1016/0024-3205(94)00677-6.

DOI:10.1016/0024-3205(94)00677-6

PMID:8065232

Abstract

Using DNA fingerprint analyses, extensive molecular heterogeneity has been found between spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY), suggesting a doubtful value of simply comparing these rats in the study of the pathogenesis of genetic hypertension. Therefore, we evaluated the genetic similarity between Dahl salt-sensitive and salt-resistant rats newly inbred by Dr.Iwai (Dahl-Iwai S and R rats), by use of DNA fingerprint analysis. Fingerprint patterns were generated by probing Hinf I- or Alu I-digested DNA with an oligonucleotide corresponded to the tandem repeat sequence of the human myoglobin 33.6 minisatellite. These fingerprint patterns were same within each strain. S and R rts shared 82 percent of the bands in Hinf I-digested DNA and 93 percent of those in Alu I-digested DNA. Although Dahl-Iwai S and R rats are more closely related than SHR and WKY rats, multiple genetic differences still exist between these Dahl strains.

摘要

通过DNA指纹分析发现，自发性高血压大鼠（SHR）和Wistar-Kyoto大鼠（WKY）之间存在广泛的分子异质性，这表明在遗传性高血压发病机制研究中简单比较这些大鼠的价值存疑。因此，我们利用DNA指纹分析评估了岩井博士新培育的Dahl盐敏感和盐抵抗大鼠（Dahl-Iwai S和R大鼠）之间的遗传相似性。用对应于人肌红蛋白33.6微卫星串联重复序列的寡核苷酸探测经Hinf I或Alu I消化的DNA，从而产生指纹图谱。每个品系内的这些指纹图谱是相同的。S大鼠和R大鼠在经Hinf I消化的DNA中共有82%的条带，在经Alu I消化的DNA中共有93%的条带。尽管Dahl-Iwai S大鼠和R大鼠的亲缘关系比SHR大鼠和WKY大鼠更近，但这些Dahl品系之间仍然存在多个遗传差异。