Greene E L, Halbert S P, Kiefer D
Int Arch Allergy Appl Immunol. 1975;48(6):764-75. doi: 10.1159/000231365.
Hyperimmune antiserum to human pancreas was exhaustively absorbed with plasma, kidney, and submaxillary gland. The resulting antiserum showed up to seven antigens in pancreas extracts by gel diffusion methods, but failed to react detectably with extracts of 17 other organs and tissues. However, this apparently "tissue-specific" anti-pancreas reagent regularly revealed two of the pancreas antigens in normal human urine concentrates. One of the latter was an anodal esterase. Although the identity of the second is not yet known, it was shown to be devoid of the following enzyme activities: amylase, catalase, glucosaminidase, glucuronidase, cystine-aminopeptidase, phosphatases and sulfatase.
用人胰腺的超免疫抗血清与血浆、肾脏和颌下腺进行彻底吸收。通过凝胶扩散法，所得抗血清在胰腺提取物中显示出多达七种抗原，但与其他17种器官和组织的提取物未检测到反应。然而，这种明显“组织特异性”的抗胰腺试剂在正常人尿浓缩物中经常显示出两种胰腺抗原。其中一种是阳极酯酶。虽然第二种抗原的身份尚不清楚，但已证明它缺乏以下酶活性：淀粉酶、过氧化氢酶、氨基葡萄糖苷酶、葡萄糖醛酸酶、胱氨酸氨基肽酶、磷酸酶和硫酸酯酶。
