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[Cloning the human cytomegalovirus immediate-early gene and expression of it in Escherichia coli].

作者信息

Klichko V I, Zvonarev A Iu, Shatalin K Iu, Kuliakina M N

出版信息

Mol Gen Mikrobiol Virusol. 1994 May-Jun(3):20-2.

PMID:8065379
Abstract

The major immediate early gene of human cytomegalovirus has been cloned. The fragment of the immediate early protein containing 351 amino acids has been fused with cro-beta-galactosidase and expressed in Escherichia coli cells. The obtained recombinant protein reacts in immunoblotting with the antibodies in human sera from patients suffering from acute cytomegalovirus infection.

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