[某些1型单纯疱疹病毒基因在大肠杆菌中的克隆与表达]
[Cloning and expression of certain herpes simplex virus type 1 genes in Escherichia coli].
作者信息
Shatalin K Iu, Zvonarev A Iu, Klichko V I, Karaseva E V, Kuliakina M N
出版信息
Mol Gen Mikrobiol Virusol. 1994 Mar-Apr(2):14-6.
Complete genes IE12, IE63, IE68, IE175, UL19, UL29 of herpes simplex virus type I or their fragments have been cloned in Escherichia coli cells. The peptides expressed were shown to be fused with cro-beta-galactosidase proteins. The recombinant proteins containing amino acid sequences of ICP4, ICP27 and ICP47, major capsid protein, and major DNA-binding protein react in immunoblotting with the anti-HSVI serum from hyperimmune rabbit. The recombinant proteins can be used for creation of diagnosticums and other scientific and practical purposes. The immunological properties of the recombinant proteins are being investigated.
单纯疱疹病毒I型的完整基因IE12、IE63、IE68、IE175、UL19、UL29或其片段已在大肠杆菌细胞中克隆。所表达的肽显示与cro-β-半乳糖苷酶蛋白融合。含有主要衣壳蛋白、主要DNA结合蛋白ICP4、ICP27和ICP47氨基酸序列的重组蛋白在免疫印迹中与来自超免疫兔的抗HSVI血清发生反应。这些重组蛋白可用于制备诊断试剂及其他科学和实际用途。目前正在研究这些重组蛋白的免疫学特性。
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