使用巨大芽孢杆菌对展青霉素进行微生物测定。
Microbiological assay of patulin, using Bacillus megaterium.
作者信息
Stott W T, Bullerman L B
出版信息
J Assoc Off Anal Chem. 1975 May;58(3):497-9.
Bacillus megaterium NRRL 1368 was found to be sensitive to patulin and a suitable test organism for an accurate, quantitative bioassay of the toxin. The optimum conditions for the test were determined. The response of the organism to patulin was found to be linear between 2 and 80 mug. When compared to thin layer chromatographic and spectrophotometric assay methods, the bioassay was found to be comparable in accuracy, but less sensitive. The test was found to be sensitive to 1.7 mug patulin. The assay is rapid (12-15 hr), simple, and inexpensive and can be used to verify the toxicity of samples, as well as to quantitatively measure patulin in samples of liquid media, apple juice, and corn.
巨大芽孢杆菌NRRL 1368被发现对展青霉素敏感，是一种用于该毒素准确、定量生物测定的合适测试生物。确定了测试的最佳条件。发现该生物对展青霉素的反应在2至80微克之间呈线性。与薄层色谱法和分光光度法测定方法相比，生物测定法在准确性上相当，但灵敏度较低。该测试对1.7微克展青霉素敏感。该测定快速（12 - 15小时）、简单且成本低廉，可用于验证样品的毒性，以及定量测定液体培养基、苹果汁和玉米样品中的展青霉素。
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