Livache T, Roget A, Dejean E, Barthet C, Bidan G, Téoule R
CIS Bio International, Laboratoire des Sondes Moléculaires, Gif-sur-Yvette, France.
Nucleic Acids Res. 1994 Aug 11;22(15):2915-21. doi: 10.1093/nar/22.15.2915.
A new methodology for the preparation of addressed DNA matrices is described. The process includes an electrochemically directed copolymerization of pyrrole and oligonucleotides bearing on their 5' end a pyrrole moiety introduced by phosphoramidite chemistry. The electro-controlled synthesis of the copolymer (poly-pyrrole) gives, in one step, a solid conducting film deposited on the surface of an electrode. The resulting polymer consists of pyrrole chains bearing covalently linked oligonucleotide. The polymer growth is limited to the electrode surface, so that it is possible to prepare a DNA matrix on a multiple electrode device by successive copolymerizations. A support bearing four oligonucleotides was used to detect three ras mutations on a synthetic DNA fragment.
本文描述了一种制备寻址DNA矩阵的新方法。该过程包括吡咯与5'端带有通过亚磷酰胺化学引入的吡咯部分的寡核苷酸的电化学定向共聚。共聚物（聚吡咯）的电控合成一步即可在电极表面沉积一层固体导电膜。所得聚合物由带有共价连接寡核苷酸的吡咯链组成。聚合物的生长限于电极表面，因此可以通过连续共聚在多电极装置上制备DNA矩阵。使用带有四种寡核苷酸的载体检测合成DNA片段上的三种ras突变。
