Livache T, Fouque B, Roget A, Marchand J, Bidan G, Téoule R, Mathis G
CIS Bio International, DIVT, Bagnols/Céze, France.
Anal Biochem. 1998 Jan 15;255(2):188-94. doi: 10.1006/abio.1997.2462.
We describe in this article an oligonucleotide array constructed on a silicon device bearing a matrix of addressable 50-microns microelectrodes. Each electrode was covered by a conducting polymer (polypyrrole) grafted by an oligonucleotide (ODN). The DNA chip was prepared by successive electrochemically addressed copolymerizations of 5' pyrrole-labeled ODN and pyrrole. Following hybridization of the biotinylated amplified sample on the chip bearing a series of probes, detection was carried out by fluorescence microscopy through an R-phycoerythrin label. This technology was successfully applied to the genotyping of hepatitis C virus in blood samples. Results show good sensitivity and a high degree of dimensional resolution.
我们在本文中描述了一种构建在带有可寻址50微米微电极矩阵的硅器件上的寡核苷酸阵列。每个电极都被通过寡核苷酸（ODN）接枝的导电聚合物（聚吡咯）覆盖。DNA芯片是通过5'吡咯标记的ODN和吡咯的连续电化学寻址共聚制备的。在带有一系列探针的芯片上对生物素化的扩增样品进行杂交后，通过R-藻红蛋白标记的荧光显微镜进行检测。该技术已成功应用于血液样本中丙型肝炎病毒的基因分型。结果显示出良好的灵敏度和高度的尺寸分辨率。
