Kim K K, Hwang K Y, Choi K D, Kang J H, Yoo O J, Suh S W
Department of Chemistry, College of Natural Sciences, Seoul National University, Korea.
Proteins. 1993 Feb;15(2):213-5. doi: 10.1002/prot.340150212.
Large crystals of arylesterase from Pseudomonas fluorescens have been grown at room temperature using ammonium sulfate as a precipitant. They grow to dimensions of 0.7 x 0.7 x 0.6 mm3 within a month. The crystals belong to the trigonal space group P3(1) (or P3(2)), with unit cell dimensions of a = 147.12 A and c = 131.08 A. The asymmetric unit seems to contain six molecules of dimeric arylesterase, with corresponding crystal volume per protein mass (VM) of 2.53 A3/Da and solvent fraction of 51.5% by volume. The crystals diffract to at least 2.2 A Bragg spacing when exposed to X-rays from a rotating-anode source. X-ray data have been collected to 2.9 A Bragg spacing from native crystals.
利用硫酸铵作为沉淀剂，在室温下培养出了荧光假单胞菌芳基酯酶的大晶体。它们在一个月内长到0.7×0.7×0.6立方毫米的尺寸。这些晶体属于三方晶系空间群P3(1)（或P3(2)），晶胞参数为a = 147.12 Å和c = 131.08 Å。不对称单元似乎包含六个二聚体芳基酯酶分子，每蛋白质质量对应的晶体体积（VM）为2.53 ų/Da，溶剂体积分数为51.5%。当暴露于旋转阳极源的X射线时，这些晶体的衍射至少可达2.2 Å的布拉格间距。已从天然晶体收集到2.9 Å布拉格间距的X射线数据。
