Vasänge-Tuominen M, Perera-Ivarsson P, Shen J, Bohlin L, Rolfsen W
Department of Pharmacy, BMC, Uppsala, Sweden.
Prostaglandins Leukot Essent Fatty Acids. 1994 May;50(5):279-84. doi: 10.1016/0952-3278(94)90167-8.
The fern Polypodium decumanum, commonly called Calaguala, has a clinically documented use in South America and Spain in the treatment of psoriasis. One of the inflammatory mediators isolated in abnormally high quantities in the psoriatic skin is leukotriene B4 (LTB4). Calaguala was tested in an in vitro model using human leukocytes for its ability to inhibit the LTB4 formation. The inhibition was found to be caused by the polyunsaturated fatty acids (PUFAs) linoleic, linolenic and arachidonic acid. IC50 values were determined for the isolated acids and compared to a group of closely related acids also commonly found in nature. The IC50 values for most acids tested were of the same magnitude (20-60 microM) except for arachidonic acid which showed stimulatory activity and 8(R) hydroxylinoleic acid which gave 30% inhibition with the highest dose tested (120 microM). The amounts of PUFAs in different Calaguala extracts were quantitatively analysed and it is concluded that the fatty acid constituents of Calaguala may contribute to the clinical effects of the extract.
蕨类植物大叶石韦，通常被称为卡拉瓜拉，在南美洲和西班牙有临床记录显示其可用于治疗银屑病。银屑病皮肤中异常大量分离出的一种炎症介质是白三烯B4（LTB4）。在体外模型中使用人白细胞测试了卡拉瓜拉抑制LTB4形成的能力。发现这种抑制是由多不饱和脂肪酸（PUFA）亚油酸、亚麻酸和花生四烯酸引起的。测定了分离出的酸的半数抑制浓度（IC50）值，并与一组在自然界中也常见的密切相关的酸进行了比较。除了显示出刺激活性的花生四烯酸和在最高测试剂量（120微摩尔）下产生30%抑制作用的8（R）-羟基亚油酸外，大多数测试酸的IC50值处于相同范围（20 - 60微摩尔）。对不同卡拉瓜拉提取物中的多不饱和脂肪酸含量进行了定量分析，得出结论：卡拉瓜拉的脂肪酸成分可能有助于提取物的临床效果。
