Chen M F, Hwang T L
Department of Surgery, Chang Gung Medical College, Taipei, Taiwan, ROC.
Proc Natl Sci Counc Repub China B. 1994 Apr;18(2):71-5.
There are conflicting data regarding the ability of the liver to regenerate after partial hepatectomy in animals and humans with cirrhosis. The purpose of this study was to document liver regeneration after partial hepatectomy in a carbon tetrachloride rat model of cirrhosis. Liver cirrhosis was produced by weekly intragastric gavage with carbon tetrachloride in 80 adult male rats. Vehicle-gavaged rats (n = 24) served as healthy controls. Liver regeneration was documented 1, 2, 3 and 7 days after partial hepatectomy on the basis of restitution of liver mass and [3H] thymidine incorporation into liver DNA. Restitution of liver mass after partial hepatectomy was significantly decreased in carbon tetrachloride-treated rats at 1 and 7 days compared with the vehicle-treated control rats. In cirrhotic rats, restitution of liver mass at 1, 2, 3 and 7 days did not differ between 33% and 70% hepatectomy. Hepatic DNA synthesis, however, was significantly impaired at 1 and 2 days in cirrhotic rats after 70% hepatectomy and also at 1 day after 33% hepatectomy compared with non-cirrhotic rats. The results of this study indicated that live regeneration did occur after partial hepatectomy in carbon tetrachloride-treated rats but was impaired in comparison with that in vehicle-treated control rats.
关于肝硬化动物和人类在部分肝切除术后肝脏的再生能力，存在相互矛盾的数据。本研究的目的是记录在四氯化碳诱导的肝硬化大鼠模型中部分肝切除术后的肝脏再生情况。通过每周给80只成年雄性大鼠灌胃四氯化碳来诱导肝硬化。灌胃赋形剂的大鼠（n = 24）作为健康对照。根据肝脏质量的恢复情况以及[3H]胸苷掺入肝脏DNA的情况，在部分肝切除术后1、2、3和7天记录肝脏再生情况。与灌胃赋形剂的对照大鼠相比，四氯化碳处理的大鼠在部分肝切除术后1天和7天肝脏质量的恢复明显降低。在肝硬化大鼠中，33%和70%肝切除术后1、2、3和7天肝脏质量的恢复没有差异。然而，与非肝硬化大鼠相比，70%肝切除术后1天和2天以及33%肝切除术后1天，肝硬化大鼠的肝脏DNA合成明显受损。本研究结果表明，在四氯化碳处理的大鼠中，部分肝切除术后确实发生了肝脏再生，但与灌胃赋形剂的对照大鼠相比，再生受到了损害。
