Hwang T L, Yu H C, Chen P C, Chen M F
Department of Surgery, Chang Gung Memorial Hospital, Chang Gung College of Medicine and Technology, Taipei, Taiwan, ROC.
Res Exp Med (Berl). 1995;195(4):201-8. doi: 10.1007/BF02576789.
The effects of hepatic stimulatory substance (HSS) on cirrhotic and non-cirrhotic rats were studied after 70% partial hepatectomy. Liver cirrhosis was produced by weekly intragastric infusion of chloroform for 12-16 weeks. The HSS was prepared by extraction from the livers of weanling mice. Rats in the experimental group were injected with 5 ml HSS after 70% partial hepatectomy, and those in the control group received normal saline. The results showed that the 3H-thymidine incorporation was higher in the HSS group 24 h after partial hepatectomy in both cirrhotic and non-cirrhotic rats, and persistently higher in the non-cirrhotic rats at 48 h. Total DNA was significantly higher in the HSS group of non-cirrhotic rats 24 and 48 h after partial hepatectomy. The restituted liver volume and weight was significantly higher in non-cirrhotic rats 48 h after partial hepatectomy, while there was no significant difference between the HSS and the control groups in the cirrhotic rats. The HSS induced significant effects on 3H-thymidine incorporation in the non-cirrhotic liver, resulting in increasing liver weight, volume and total DNA 48 h after partial hepatectomy. In cirrhotic rats, the 3H-thymidine incorporation was higher in the HSS group at 24 h after partial hepatectomy, though not showing any increase at 48 h, but the regeneration of liver weight, volume and total DNA at 48 h showed no difference between the HSS group and the control group.
在70%肝部分切除术后，研究了肝刺激物质（HSS）对肝硬化和非肝硬化大鼠的影响。通过每周经胃内注入氯仿12 - 16周来诱导肝硬化。HSS通过从断乳小鼠肝脏中提取制备。实验组大鼠在70%肝部分切除术后注射5 ml HSS，对照组大鼠则接受生理盐水。结果显示，在肝部分切除术后24小时，肝硬化和非肝硬化大鼠的HSS组中3H - 胸苷掺入率均较高，并且在48小时时非肝硬化大鼠中持续较高。在肝部分切除术后24小时和48小时，非肝硬化大鼠的HSS组中总DNA显著更高。在肝部分切除术后48小时，非肝硬化大鼠的肝脏再生体积和重量显著更高，而在肝硬化大鼠中HSS组和对照组之间没有显著差异。HSS对非肝硬化肝脏中的3H - 胸苷掺入有显著影响，导致在肝部分切除术后48小时肝脏重量、体积和总DNA增加。在肝硬化大鼠中，肝部分切除术后24小时HSS组的3H - 胸苷掺入率较高，尽管在48小时时没有显示出任何增加，但在48小时时HSS组和对照组之间肝脏重量、体积和总DNA的再生没有差异。
