Sample A K, Czuprynski C J
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison 53706.
Vet Immunol Immunopathol. 1994 May;41(1-2):165-72. doi: 10.1016/0165-2427(94)90065-5.
Previous studies have indicated that certain types of bovine cells preferentially respond to bovine interleukin-1 (IL-1) compared with IL-1 from other mammalian species. In this study, we demonstrate that bovine neutrophils undergo a substantially greater luminol-dependent chemiluminescence response to bovine IL-1 beta than to human IL-1 alpha, human IL-1 beta, or murine IL-1 alpha. Likewise, approximately 1000-fold less bovine IL-1 beta than human IL-1 beta was required to prime bovine neutrophils for an enhanced luminol-dependent chemiluminescence response to opsonized zymosan particles. A recombinant human IL-1 receptor antagonist was a potent inhibitor of bovine neutrophil stimulation and priming by homologous and heterologous IL-1. These data indicate a hierarchy amongst members of the IL-1 family in terms of their relative abilities to modulate the functional activation of bovine neutrophils.
先前的研究表明，与其他哺乳动物来源的白细胞介素-1（IL-1）相比，某些类型的牛细胞对牛IL-1有优先反应。在本研究中，我们证明，与人类IL-1α、人类IL-1β或小鼠IL-1α相比，牛中性粒细胞对牛IL-1β产生的鲁米诺依赖性化学发光反应要大得多。同样，与人类IL-1β相比，引发牛中性粒细胞对调理酵母聚糖颗粒产生增强的鲁米诺依赖性化学发光反应所需的牛IL-1β量约少1000倍。重组人IL-1受体拮抗剂是同源和异源IL-1刺激和引发牛中性粒细胞的有效抑制剂。这些数据表明，就调节牛中性粒细胞功能激活的相对能力而言，IL-1家族成员之间存在等级关系。
