Sample A K, Czuprynski C J
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison 53706.
J Leukoc Biol. 1991 Feb;49(2):107-15. doi: 10.1002/jlb.49.2.107.
We have examined the in vitro effects of two recombinant human monokines, interleukin-1 alpha (rHuIL-1 alpha) and tumor necrosis factor alpha (rHuTNF alpha), on bovine neutrophil functions. Both rHuIL-1 alpha (10 to 1,000 ng/ml) and rHuTNF alpha (5 to 50 ng/ml) directly stimulated the oxidative burst of bovine neutrophils as measured by Luminol-dependent chemiluminescence, superoxide anion generation, and hydrogen peroxide production. In addition, both rHuIL-1 alpha (1 to 1,000 ng/ml) and rHuTNF alpha (0.5 to 50 ng/ml) primed bovine neutrophils for an enhanced oxidative burst to subsequent stimulation with opsonized zymosan. Neutrophils pre-treated with either monokine exhibited an earlier, as well as stronger, zymosan-stimulated Luminol-dependent chemiluminescence response, as compared to untreated neutrophils. Exposure of bovine neutrophils to combinations of suboptimal doses of rHuIL-1 alpha (10 and 100 ng/ml) and rHuTNF alpha (0.5 and 5 ng/ml) resulted in a synergistic stimulation of Luminol-dependent chemiluminescence, whereas, no synergism was observed when using optimal doses of each monokine. Pre-incubation of bovine neutrophils with an optimal concentration of recombinant bovine interferon gamma (100 U/ml), and either rHuIL-1 alpha or rHuTNF alpha, further augmented the maximal oxidative response of neutrophils stimulated with opsonized zymosan. Bovine neutrophils released both primary and secondary granules in response to rHuIL-1 alpha and rHuTNF alpha, and also exhibited enhanced adherence in the presence of either monokine.
我们研究了两种重组人单核因子，即白细胞介素 -1α（rHuIL -1α）和肿瘤坏死因子α（rHuTNFα）对牛中性粒细胞功能的体外作用。rHuIL -1α（10至1000 ng/ml）和rHuTNFα（5至50 ng/ml）均能直接刺激牛中性粒细胞的氧化爆发，这可通过鲁米诺依赖性化学发光、超氧阴离子生成和过氧化氢产生来测定。此外，rHuIL -1α（1至1000 ng/ml）和rHuTNFα（0.5至50 ng/ml）均能使牛中性粒细胞致敏，使其在随后用调理酵母聚糖刺激时氧化爆发增强。与未处理的中性粒细胞相比，用任一单核因子预处理的中性粒细胞对酵母聚糖刺激的鲁米诺依赖性化学发光反应出现得更早且更强。将牛中性粒细胞暴露于次优剂量的rHuIL -1α（10和100 ng/ml）和rHuTNFα（0.5和5 ng/ml）组合中，会导致鲁米诺依赖性化学发光的协同刺激，而当使用每种单核因子的最佳剂量时未观察到协同作用。用最佳浓度的重组牛干扰素γ（100 U/ml）与rHuIL -1α或rHuTNFα对牛中性粒细胞进行预孵育，可进一步增强经调理酵母聚糖刺激的中性粒细胞的最大氧化反应。牛中性粒细胞对rHuIL -1α和rHuTNFα的反应是释放初级和次级颗粒，并且在存在任一单核因子时也表现出增强的黏附性。
