Kravtsov A N, Tynianova V I, Ziuzina V P
Zh Mikrobiol Epidemiol Immunobiol. 1993 Jul-Aug(4):3-6.
The incubation of Y. pestis, the causative agent of plague, in hemolyzed human red blood cells at 37 degrees C for 3 hours enhanced the virulence of vaccine strain EV76 in white mice and guinea pigs and decreased the LD50 of attenuated strains. The phenomenon of increased virulence has been observed only in case of the combined injection of bacteria with the incubation medium. The virulence of Y. pestis cells, introduced alone after such treatment, has proved to remain at the same level.
鼠疫病原体鼠疫耶尔森菌在37摄氏度下于溶血的人红细胞中孵育3小时，可增强疫苗株EV76对小白鼠和豚鼠的毒力，并降低减毒株的半数致死量（LD50）。仅在将细菌与孵育培养基联合注射的情况下才观察到毒力增强现象。经此类处理后单独引入的鼠疫耶尔森菌细胞的毒力已证明保持在同一水平。
