Cheung T K, Cunningham J M, Webb M J, Goellner J R, Kovach J S
Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, N.T.
Anticancer Res. 1994 May-Jun;14(3B):1335-8.
We explored the value of p53 immunostaining in detection of malignant cells in cytologic analyses in ovarian cancer patients. Ninety-six percent of 23 tumor touch preparations had nuclear staining with PAb240: 44% had nuclear only, 26% had both nuclear and cytoplasmic staining, and 26% had cytoplasmic staining alone. Eight of 20 cytospins of peritoneal washings from ovarian cancer patients were PAb240 positive, 6 had cytoplasmic, and two had nuclear staining. Cytospins of fluid from 3/15 patients with no malignancy revealed cytoplasmic staining of mesothelial cells. The lack of concordance between staining of primary tumors and cytospins of peritoneal washings and the apparent reactivity in some benign samples may limit the utility of immunohistochemical detection of malignant cells in the peritoneal fluid of ovarian cancer patients.
我们探讨了p53免疫染色在卵巢癌患者细胞学分析中检测恶性细胞的价值。23份肿瘤触片中有96%经PAb240染色后细胞核呈阳性：44%仅细胞核呈阳性，26%细胞核和细胞质均呈阳性，26%仅细胞质呈阳性。20份卵巢癌患者腹腔冲洗液的细胞涂片中有8份PAb240呈阳性，6份细胞质呈阳性，2份细胞核呈阳性。15例无恶性肿瘤患者中有3例的液体细胞涂片显示间皮细胞细胞质呈阳性。原发性肿瘤染色与腹腔冲洗液细胞涂片之间缺乏一致性，以及一些良性样本中出现明显反应性，可能会限制免疫组化检测卵巢癌患者腹腔液中恶性细胞的实用性。
