Fry J M, Allen J G, Speijers E J, Roberts W D
Animal Health Laboratories, Department of Agriculture, South Perth, Western Australia.
Aust Vet J. 1994 May;71(5):146-50. doi: 10.1111/j.1751-0813.1994.tb03369.x.
The diagnostic performance of plasma tests for muscle enzymes was measured in sheep from flocks affected by clinical and sub-clinical nutritional myopathy. Parallel combinations of tests for creatine kinase (CK), alanine amino transferase (ALT), aspartate amino transferase (AST) and lactate dehydrogenase had higher diagnostic sensitivity than CK alone. The enzymes ALT and AST showed the highest correlation with the degree of muscle damage. A parallel combination of tests for plasma CK and ALT as well as tests for plasma alpha-tocopherol and red cell glutathione peroxidase are recommended for the diagnosis of nutritional myopathy and a decision on the appropriate treatment. The number of false negative results based on a diagnosis from the microscopic examination of single muscles was higher than for the parallel combination of tests. The number of false negatives was highest for the vastus intermedius and lowest for the tensor fascia lata. Diagnosis using a panel of blood tests has the advantages of overcoming problems of inadequate muscle sampling, a larger number of sheep in the flock can be tested and a more rapid diagnosis can be obtained.
在受临床和亚临床营养性肌病影响的羊群中，对绵羊进行了血浆肌肉酶检测的诊断性能评估。肌酸激酶（CK）、丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）和乳酸脱氢酶检测的平行组合比单独检测CK具有更高的诊断敏感性。ALT和AST这两种酶与肌肉损伤程度的相关性最高。建议采用血浆CK和ALT检测的平行组合以及血浆α-生育酚和红细胞谷胱甘肽过氧化物酶检测来诊断营养性肌病并决定适当的治疗方案。基于单块肌肉显微镜检查诊断得出的假阴性结果数量高于检测的平行组合。股中间肌的假阴性数量最多，阔筋膜张肌的假阴性数量最少。使用一组血液检测进行诊断具有克服肌肉采样不足问题的优点，可以检测更多的羊群，并且能够更快地获得诊断结果。
