Kass G E, Webb D L, Chow S C, Llopis J, Berggren P O
Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.
Biochem J. 1994 Aug 15;302 ( Pt 1)(Pt 1):5-9. doi: 10.1042/bj3020005.
In single Fura-2 ester-loaded hepatocytes, stimulation by vasopressin, but not emptying of the agonist-sensitive Ca2+ store by 2,5-di-(t-butyl)hydroquinone, resulted in an increase in the rate of Fura-2 fluorescence-quenching by Mn2+. Similarly, in cells microinjected with Fura-2 salt, vasopressin stimulated Mn2+ entry while 2,5-di-(t-butyl)hydroquinone or thapsigargin did not. The pattern of Fura-2 quenching by Mn2+ only correlated with the movement of Mn2+ across the plasma membrane.
在单个负载Fura-2酯的肝细胞中，血管加压素刺激可导致Mn2+引起的Fura-2荧光猝灭速率增加，而2,5-二（叔丁基）对苯二酚排空激动剂敏感的Ca2+储存库则不会。同样，在显微注射Fura-2盐的细胞中，血管加压素刺激Mn2+内流，而2,5-二（叔丁基）对苯二酚或毒胡萝卜素则无此作用。Mn2+引起的Fura-2猝灭模式仅与Mn2+跨质膜的移动相关。
