Lederer T, Rippon J, Baldwin S, Pachman L M
Mycopathologia. 1975 Feb 28;55(1):65-9. doi: 10.1007/BF00467094.
Parenteral alimentation is often complicated by Candida albicans infection which may be fatal. This study investigated the effect of alimentation fluid (Aminosol) on C. albicans' growth in vitro. It was found that concentrated Aminosol (1400 millisomoles) maintained C. albicans in a viable state but inhibited replication. Dilution of alimentation fluid to physiological concentrations (300 milliosmoles) with either saline or aged pooled normal sera promoted in vitro growth of C. albicans which was equivalent to that obtained in BHI broth and was slightly less than that obtained in Sabouraud's broth. The effects of fresh sera with full complement activity were also investigated. In fresh sera appropriately diluted with physiological saline, some clumping of the yeasts was observed and all formed germ tubes. Growth as defined by budding or the formation of hyphae was inhibited. When Aminosol was diluted to 300 milliosomoles with fresh sera, all yeasts were noted to be in clumps with germ tubes as well as continually growing hyphae. Growth was approximately equal to that seen in Aminosol similarly diluted with saline.
肠外营养常并发白色念珠菌感染，这可能是致命的。本研究调查了营养液（氨基醇）对白色念珠菌体外生长的影响。结果发现，浓缩的氨基醇（1400毫渗量）可使白色念珠菌维持存活状态，但抑制其复制。用生理盐水或陈旧混合正常血清将营养液稀释至生理浓度（300毫渗量）可促进白色念珠菌的体外生长，其生长情况与在脑心浸液肉汤中获得的相当，略低于在沙氏肉汤中获得的。还研究了具有完全补体活性的新鲜血清的作用。在用生理盐水适当稀释的新鲜血清中，观察到一些酵母细胞聚集，并且所有酵母都形成了芽管。出芽或形成菌丝所定义的生长受到抑制。当用新鲜血清将氨基醇稀释至300毫渗量时，所有酵母都被观察到聚集并带有芽管以及不断生长的菌丝。生长情况与用生理盐水类似稀释的氨基醇中的生长情况大致相同。
