Liu Y L, Wang J F, Qiu B S, Zhao S Z, Tian B
Institute of Microbiology, Academia Sinica, Beijing, PRC.
Sci China B. 1994 Jan;37(1):37-41.
Expression of Human hepatitis B virus surface antigen (HBsAg) gene in plant was reported for the first time. The recombinant plasmid pRoKII-HBsAg was constructed by inserting HBsAg gene into the downstream of CaMV 35S promoter of binary vector pRoKII and then introduced into Agrobacterium tumefaciens LBA4404. The kanamycin-resistant plants were obtained by Agrobacterium-mediated transformation system. It was shown that HBsAg gene was expressed in transgenic tobacco plants and their progenies by ELISA. The spherical particles of psi 22 nm is the leaf extract of transgenic tobacco were observed by immunosorbent electron microscopy.
首次报道了人类乙型肝炎病毒表面抗原（HBsAg）基因在植物中的表达。通过将HBsAg基因插入双元载体pRoKII的CaMV 35S启动子下游构建重组质粒pRoKII-HBsAg，然后将其导入根癌农杆菌LBA4404。通过农杆菌介导的转化系统获得了卡那霉素抗性植株。通过ELISA表明HBsAg基因在转基因烟草植株及其后代中表达。通过免疫吸附电子显微镜观察到转基因烟草叶片提取物中有22 nm的球形颗粒。
