乙型肝炎病毒表面抗原在转基因植物细胞和组织培养中的组织特异性表达
Tissue specific expression of hepatitis B virus surface antigen in transgenic plant cells and tissue culture.
作者信息
Rukavtsova E B, Abramikhina T V, Shulga N Ya, Bykov V A, Bur'yanov Ya I
机构信息
1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Pushchino Branch, Russian Academy of Sciences, pr. Nauki 6, Pushchino, Moscow oblast, 142290 Russia.
All-Russian Institute of Medicinal and Aromatic Plants, ul. Grina 7, Moscow, 113628 Russia.
出版信息
Russ J Plant Physiol. 2007;54(6):770-775. doi: 10.1134/S1021443707060088.
The tobacco plants ( L.) carrying the gene controlled by (Aocs)AmasPmas, the hybrid promoter that includes regulatory elements of the agrobacterial octopine and mannopine synthase genes, as well as plants controlled by the same promoter and , maize alcohol dehydrogenase gene intron were obtained. The presence of the gene intron did not significantly change the level of expression of the gene in plants. The analysis of expression of hepatitis B virus surface antigen (HBs-antigen) in transformed plants expressing the under the control of different promoters was made. The level of HBs-antigen in plants carrying the gene controlled by (Aocs)AmasPmas, the hybrid agrobacterium-derived promoter, was the highest in roots and made up to 0.01% of total amount of soluble protein. The level of HBs-antigen in plants carrying the gene controlled by the dual 35S RNA cauliflower mosaic virus promoter was the same in all organs of the plant and made up to 0.06% of the total amount of soluble protein. Hairy root and callus cultures of plants carrying the gene and expressing the HBs-antigen were obtained.
获得了携带由（Aocs）AmasPmas控制的基因的烟草植株，（Aocs）AmasPmas是一种杂种启动子，包含农杆菌章鱼碱和甘露碱合成酶基因的调控元件，还获得了由相同启动子和玉米醇脱氢酶基因内含子控制的植株。该基因内含子的存在并未显著改变该基因在植株中的表达水平。对在不同启动子控制下表达该基因的转化植株中乙型肝炎病毒表面抗原（HBs抗原）的表达进行了分析。携带由杂种农杆菌衍生启动子（Aocs）AmasPmas控制的基因的植株中，HBs抗原在根中的水平最高，占可溶性蛋白总量的0.01%。携带由双35S RNA花椰菜花叶病毒启动子控制的基因的植株中，HBs抗原在植株所有器官中的水平相同，占可溶性蛋白总量的0.06%。获得了携带该基因并表达HBs抗原的植株的毛状根和愈伤组织培养物。
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