Reynolds E R, Yacynych A M
Department of Chemistry, Rutgers, State University of New Jersey, New Brunswick 08903.
Biosens Bioelectron. 1994;9(4-5):283-93. doi: 10.1016/0956-5663(94)80025-1.
Miniaturized glucose biosensors were constructed from disk-shaped ultramicroelectrodes (UMEs) (diameter = 10 microns and 25 microns), cylindrical UMEs (diameter = 25 microns), and platinum black modified UMEs. Glucose oxidase (E.C.1.1.3.4) was immobilized by glutaraldehyde cross-linking. All ultramicrobiosensors (UMBs) had response times (100% response) of less than 1 min, and had linear response over the human clinical range for glucose (3-7 mM). Response to glucose was linear over a greater concentration range at cylindrical UMBs, compared to similarly prepared disk-shaped UMBs, typically from 3-20 mM glucose. Thin, electrochemically polymerized films were used to prevent signals due to interfering species. A variety of different films, and electropolymerization conditions were examined. Poly(1,3-diaminobenzene) (1,3-DAB) was found to be the most effective at preventing signals due to interferents. Poly(1,3-DAB) was used to protect the biosensor from fouling. A 25 microns biosensor, with poly(1,3-DAB), was used for the direct measurement of glucose in complex samples.
微型葡萄糖生物传感器由盘状超微电极（UME）（直径分别为10微米和25微米）、圆柱形UME（直径为25微米）以及铂黑修饰的UME构建而成。葡萄糖氧化酶（E.C.1.1.3.4）通过戊二醛交联固定。所有超微生物传感器（UMB）的响应时间（达到100%响应）均小于1分钟，并且在人体临床葡萄糖浓度范围（3 - 7 mM）内具有线性响应。与同样制备的盘状UMB相比，圆柱形UMB对葡萄糖的响应在更大的浓度范围内呈线性，通常为3 - 20 mM葡萄糖。采用薄的电化学聚合膜来防止干扰物质产生信号。研究了多种不同的膜以及电聚合条件。发现聚（1,3 - 二氨基苯）（1,3 - DAB）在防止干扰物质产生信号方面最为有效。聚（1,3 - DAB）用于保护生物传感器不被污染。一个带有聚（1,3 - DAB）的25微米生物传感器用于直接测量复杂样品中的葡萄糖。
