Expression and localization of hepatocyte growth factor in rat submandibular gland.

By combination of in situ hybridization and immunohistochemical techniques, the expression of hepatocyte growth factor (HGF) was demonstrated in the submandibular gland of rats. Both the mRNA signal and immunoreactivity for HGF were localized exclusively to the epithelial cells of granular convoluted tubules, whereas they were absent from the other components of the submandibular gland. In the granular convoluted tubule cells, HGF-immunoreactivity was localized to the apical secretory granules, which was further substantiated by immunoelectron microscopy. These results added HGF to the list of many growth factors that are produced in the rat submandibular gland and secreted into the saliva.