Amano O, Yoshitake Y, Nishikawa K, Iseki S
Department of Anatomy, School of Medicine, Kanazawa University, Ishikawa, Japan.
Cell Tissue Res. 1993 Sep;273(3):467-74. doi: 10.1007/BF00333701.
We studied the occurrence and localization of basic fibroblast growth factor (bFGF) in rat salivary glands using a specific monoclonal antibody. It was shown that the extract of rat salivary glands has a pronounced stimulatory activity on the growth of bovine capillary endothelial cells, which is blocked by the addition of an antibody against bFGF. The concentration of bFGF in the submandibular/sublingual gland, as determined by radioimmunoassay, was approximately 80% that in the brain. Immunocytochemistry revealed bFGF-immunoreactivity localized primarily in the epithelial cells lining the striated ducts and excretory ducts of the parotid, sublingual and submandibular glands. In addition, intense bFGF-immunoreactivity was observed in the granular convoluted tubule of the submandibular gland, localized predominantly in the agranular pillar cells, which lay in small numbers among the majority of weakly immunostained cells containing many apical secretory granules. At the electron-microscopic level, the immunoreactive material was distributed diffusely in the cytoplasmic matrix and nuclei of all immunoreactive cells, whereas it was absent from all cytoplasmic organelles including the secretory granules. These results indicate that bFGF is localized in different cellular and subcellular compartments from those of other growth factors in the duct system of rat salivary glands.
我们使用一种特异性单克隆抗体研究了碱性成纤维细胞生长因子（bFGF）在大鼠唾液腺中的分布及定位情况。结果显示，大鼠唾液腺提取物对牛毛细血管内皮细胞的生长具有显著的刺激活性，而添加抗bFGF抗体可阻断这种活性。通过放射免疫分析法测定，下颌下腺/舌下腺中bFGF的浓度约为脑内浓度的80%。免疫细胞化学显示，bFGF免疫反应性主要定位于腮腺、舌下腺和下颌下腺的纹状管及排泄管内衬的上皮细胞中。此外，在下颌下腺的颗粒曲管中观察到强烈的bFGF免疫反应性，主要定位于无颗粒柱状细胞，这些细胞数量较少，位于大多数弱免疫染色且含有许多顶端分泌颗粒的细胞之中。在电子显微镜水平上，免疫反应物质弥散分布于所有免疫反应细胞的细胞质基质和细胞核中，而包括分泌颗粒在内的所有细胞质细胞器中均未发现。这些结果表明，在大鼠唾液腺导管系统中，bFGF的定位与其他生长因子的定位处于不同的细胞和亚细胞区室。
