Gel-permeation liquid chromatographic method for determination of polymerized triglycerides in oils and fats: summary of collaborative study.

The IUPAC Commission on Oils, Fats and Derivatives undertook development of a method and collaborative study for determination of polymerized triglycerides in oils and fats. Two collaborative studies were carried out by IUPAC in 1985 and 1986. Both studies consisted of 4 materials, including animal fat, frying oil with average content of polymers, and blends of frying oil containing varying amounts of polymers. Thirteen and 17 laboratories were included in the first and second IUPAC collaborative studies, respectively. Another collaborative study conducted by inspectorate for Health Protection, Food Inspection Service, The Netherlands, included 13 laboratories. Six heat-processed fat samples (3 pairs of split samples) containing 14-28% (w/w) of polymerized triglycerides were studied. The method was adopted by AOAC INTERNATIONAL as an IUPAC-AOCS-AOAC method.