Miranda M, Amicarelli F, Poma A, Ragnelli A M, Scirri C, Aimola P P, Masciocco L, Bonfigli A, Zarivi O
Department of Cell Biology and Physiology, University of L'Aquila, Coppito, Italy.
Biochem Mol Biol Int. 1994 Apr;32(5):913-22.
This work studies the phenotype changes, relating to pigment expression, of a human melanoma cell line. The phenotypic instabilities and proliferation rates are correlated with the production and release in the cell culture medium of active oxygen species and melanin synthesis intermediates. The proliferation rates versus L-tyrosine concentration in the culture media are investigated: a decrease is found when high L-tyrosine is added to the medium. This would be consistent with the release of cytotoxic and/or genotoxic species by melanoma cells. The morphology of melanoma melanosomes is coherent with the leakage of cytotoxic and genotoxic species produced during melanin synthesis.
这项工作研究了一种人类黑色素瘤细胞系与色素表达相关的表型变化。表型不稳定性和增殖率与细胞培养基中活性氧物种和黑色素合成中间体的产生及释放相关。研究了培养基中增殖率与L-酪氨酸浓度的关系：当向培养基中添加高浓度L-酪氨酸时，发现增殖率下降。这与黑色素瘤细胞释放细胞毒性和/或基因毒性物质是一致的。黑色素瘤黑素小体的形态与黑色素合成过程中产生的细胞毒性和基因毒性物质的泄漏相一致。
