Miyamoto T, Takeuchi A, Hayashi H, Onozaki K
Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Biochem Mol Biol Int. 1994 Apr;32(5):973-81.
Cell extracts from a variety of cell lines, myeloid, T, B, mastocytoma, fibroblast, melanoma and breast carcinoma of human, mouse and guinea pig promoted the growth of a wide variety of cell types, namely human myeloid cells HL-60, human B cells Daudi, human melanoma cells A375-C6, mouse transformed-fibroblast cells L929, human myelomonocytic cells THP-1. Among them, the activities in extracts from U937, A375-C6 and Daudi were characterized because these extracts exhibited much more potent activity. These growth promoting activities were acid-labile, sensitive to 2-mercaptoethanol and to heat treatment at 50C or 70C for 5 min. The activities were also sensitive to proteases indicating the proteinous nature of these active entities. The molecular weight of activities from A375 and Daudi cells were estimated to be 100,000-150,000 daltons by gel filtration high performance liquid chromatography, while that from U937 cells was 60,000-70,000 daltons. The isoelectric point of these activities were 5.5-6.5.
来自多种细胞系（包括人、小鼠和豚鼠的髓系、T细胞、B细胞、肥大细胞瘤、成纤维细胞、黑色素瘤和乳腺癌细胞系）的细胞提取物促进了多种细胞类型的生长，即人髓系细胞HL-60、人B细胞Daudi、人黑色素瘤细胞A375-C6、小鼠转化成纤维细胞L929、人骨髓单核细胞THP-1。其中，对U937、A375-C6和Daudi细胞提取物的活性进行了表征，因为这些提取物表现出更强的活性。这些生长促进活性对酸不稳定，对2-巯基乙醇以及在50℃或70℃下热处理5分钟敏感。这些活性对蛋白酶也敏感，表明这些活性实体具有蛋白质性质。通过凝胶过滤高效液相色谱法估计，A375和Daudi细胞活性的分子量为100,000 - 150,000道尔顿，而U937细胞活性的分子量为60,000 - 70,000道尔顿。这些活性的等电点为5.5 - 6.5。
