源自灰树花（舞茸）的蛋白聚糖单克隆抗体。

Monoclonal antibody to proteoglycan derived from Grifola frondosa (Maitake).

作者信息

Hirata A, Adachi Y, Itoh W, Komoda M, Tabata K, Sugawara I

机构信息

Research Laboratory, Taito Co., Ltd., Kobe, Japan.

出版信息

Biol Pharm Bull. 1994 Apr;17(4):539-42. doi: 10.1248/bpb.17.539.

DOI:10.1248/bpb.17.539

PMID:8069265

Abstract

A murine monoclonal antibody (MAb) was prepared by immunizing BALB/c mice with a proteoglycan fraction derived from Grifola frondosa (Maitake mushroom), followed by the hybridization of spleen cells with mouse myeloma cells. The MAb (subclass; Ig G2b), designated MPG2, reacted with schizophyllan (SPG), curdlan, scleroglucan, laminarin and lentinan, but not with dextran, pullulan, mannan and xylan. Immunohistochemistry (ABC-GO method) showed that MAb MPG2 reacted with lysosomal proteoglycan and (1-->6)-beta-branched laminaritriose taken up by rabbit peritoneal macrophages. These results suggest that this MAb may recognize mainly (1-->3)-beta-D-glucan, and may be useful for determining the immunological properties of Grifola frondosa-derived proteoglycan.

摘要

用灰树花（舞茸）来源的蛋白聚糖组分免疫BALB/c小鼠，然后将脾细胞与小鼠骨髓瘤细胞杂交，制备了一种鼠单克隆抗体（MAb）。该单克隆抗体（亚类：Ig G2b）命名为MPG2，与裂褶菌多糖（SPG）、凝胶多糖、硬葡聚糖、海带多糖和香菇多糖反应，但不与葡聚糖、支链淀粉、甘露聚糖和木聚糖反应。免疫组织化学（ABC-GO法）显示，单克隆抗体MPG2与兔腹膜巨噬细胞摄取的溶酶体蛋白聚糖和（1→6）-β-分支海带三糖反应。这些结果表明，该单克隆抗体可能主要识别（1→3）-β-D-葡聚糖，可用于确定灰树花来源蛋白聚糖的免疫学特性。