使用抗寡糖（G4）单克隆抗体进行表位分析。
Epitope analysis using anti-oligosaccharide (G4) monoclonal antibody.
作者信息
Hirata A, Komoda M, Itoh W, Tabata K, Sugawara I
机构信息
Research Laboratory, Taito Co., Ltd., Hyogo, Japan.
出版信息
Microbiol Immunol. 1995;39(1):43-7. doi: 10.1111/j.1348-0421.1995.tb02166.x.
A murine monoclonal antibody recognizing (1-->6)-beta-D-glucopyranosyl laminaritriose (G4) was prepared by immunizing BALB/c mice with G4-bovine serum albumin conjugate and fusing the splenocytes with mouse myeloma cells. The monoclonal antibody (IgM) provoked by the cloned cells showed low reactivity with schizophyllan, an antitumor polysaccharide, but notable reactivity with some low-molecular-weight schizophyllans. This antibody was useful for determination of the epitope of several polysaccharides. The extent of reactivity of this monoclonal antibody was related only to the molecular weight of schizophyllan.
通过用(1→6)-β-D-吡喃葡萄糖基层孔三糖（G4）-牛血清白蛋白偶联物免疫BALB/c小鼠，并将脾细胞与小鼠骨髓瘤细胞融合，制备了一种识别(1→6)-β-D-吡喃葡萄糖基层孔三糖（G4）的鼠单克隆抗体。克隆细胞产生的单克隆抗体（IgM）与抗肿瘤多糖裂褶菌多糖反应性较低，但与一些低分子量裂褶菌多糖有显著反应性。该抗体可用于确定几种多糖的表位。这种单克隆抗体的反应程度仅与裂褶菌多糖的分子量有关。
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