Loones M T, Amirand C, Debey P, Lacroix J C, Vigny P
Laboratoire de Génétique du Dévelopment, Université P. et M. Curie, Paris, France.
Chromosome Res. 1994 May;2(3):235-44. doi: 10.1007/BF01553324.
The mitotic Z and W sex chromosomes in Pleurodeles seem to be identical. Earlier morphological and molecular analyses of lampbrush paired chromosomes in the female meiosis showed clearly that 20% of the chromosomal length located in the middle part of the sex bivalent (bivalent IV) is heteromorphic. We investigated here the base content and composition of the DNA axes in the heteromorphic region by quantitative fluorescence imaging using various base-specific (DAPI, Hoechst 33342 and chromo-mycin A3) or base-nonspecific (ethidium bromide) fluorescent DNA probes. Our results show a significantly higher percentage of AT bases in Z than in W differential sectors. In addition the entire base content of Z appears slightly higher than that of W.
美西螈有丝分裂期的Z和W性染色体似乎是相同的。早期对雌性减数分裂中灯刷配对染色体的形态学和分子分析清楚地表明，位于性二价体（二价体IV）中部的20%染色体长度是异形的。我们在此使用各种碱基特异性（4',6-二脒基-2-苯基吲哚、 Hoechst 33342和嗜铬菌素A3）或碱基非特异性（溴化乙锭）荧光DNA探针，通过定量荧光成像研究了异形区域中DNA轴的碱基含量和组成。我们的结果显示，Z染色体的AT碱基百分比显著高于W染色体的差异区段。此外，Z染色体的整体碱基含量似乎略高于W染色体。
