Yoshikawa T, Nanba T, Kato H, Hori K, Inamoto T, Kumagai S, Yodoi J
Department of Biological Responses, Faculty of Medicine, Kyoto University, Japan.
Immunomethods. 1994 Feb;4(1):65-71. doi: 10.1006/immu.1994.1008.
The low-affinity Fc receptor for IgE (Fc epsilon RII/CD23) and its soluble form (sCD23, IgE-binding factor) have multiple functions, and enhanced levels of these are associated with various immunological diseases. We established two sensitive ELISA systems using enzyme-conjugated mAb and biotinylated mAb. The detection limits of the ELISA systems were 0.03 and 1.0 ng/ml, which showed good correlation in the range 1.0-10 ng/ml. In the ELISA system using enzyme-conjugated mAb, the average sCD23 concentration in 303 normal healthy volunteers was 1.4 +/- 0.3 ng/ml. In the ELISA system using biotinylated mAb, sCD23 levels in normal healthy volunteers showed almost the same values. In patients with autoimmune diseases such as rheumatoid arthritis, systemic lupus erythematosus, Sjögren syndrome, progressive systemic sclerosis, and mixed connective tissue disease, the sCD23 levels were significantly higher than those in normal individuals. Furthermore, in Epstein-Barr virus-related disorders after liver transplantation with immunosuppression, plasma levels of sCD23 rapidly increased to more than 12 ng/ml when clinical symptoms were evident. In addition, the sCD23 values remained high, although elevated GOT levels gradually decreased to standard values and EBV hepatitis improved. These data suggest that sCD23 levels are a sensitive marker of autoimmune diseases and EBV-related disorders in addition to allergic disorders. The ELISA system for sCD23 may be an additional diagnostic tool in estimating the clinical courses of these diseases.
IgE的低亲和力Fc受体（FcεRII/CD23）及其可溶性形式（sCD23，IgE结合因子）具有多种功能，其水平升高与多种免疫性疾病相关。我们使用酶联单克隆抗体和生物素化单克隆抗体建立了两种灵敏的ELISA系统。这两种ELISA系统的检测限分别为0.03和1.0 ng/ml，在1.0 - 10 ng/ml范围内显示出良好的相关性。在使用酶联单克隆抗体的ELISA系统中，303名正常健康志愿者的平均sCD23浓度为1.4±0.3 ng/ml。在使用生物素化单克隆抗体的ELISA系统中，正常健康志愿者的sCD23水平显示出几乎相同的值。在患有类风湿性关节炎、系统性红斑狼疮、干燥综合征、进行性系统性硬化症和混合性结缔组织病等自身免疫性疾病的患者中，sCD23水平显著高于正常个体。此外，在肝移植后接受免疫抑制的爱泼斯坦 - 巴尔病毒相关疾病中，当临床症状明显时，sCD23的血浆水平迅速升高至超过12 ng/ml。另外，尽管升高的谷草转氨酶水平逐渐降至标准值且EBV肝炎有所改善，但sCD23值仍保持较高水平。这些数据表明，sCD23水平除了是过敏性疾病的敏感标志物外，也是自身免疫性疾病和EBV相关疾病的敏感标志物。用于sCD23的ELISA系统可能是评估这些疾病临床病程的一种额外诊断工具。
