Kounadi E, Tzaphlidou M, Glaros D
Laboratory of Medical Physics, Medical School, University of Ioannina, Greece.
Micron. 1994;25(3):233-40. doi: 10.1016/0968-4328(94)90028-0.
The structure of mouse skin collagen fibrils after treatment with lithium chloride at doses of 1.5 and 6 meq/kg of body weight was studied by electron microscopy. Animals were sacrificed 1 day, 1, 2 and 6 months after the end of a 30 consecutive days experimental period. With the first dose, which is commonly used clinically, although there were areas with normal collagen fibrils there were also regions where the characteristic parallel packing of fibrils was lost completely or it was preserved for a part of the area. With high doses, in most regions the normal packing of fibrils was replaced by an anarchic arrangement. In both cases, collagen fibrils had a marked decrease in mean diameter and showed a higher degree of variability in width and shape than collagen fibrils from controls. Also, many clusters of abnormal fibrils were found with a diameter of up to 260 nm vs 109 nm and a highly irregular outline when viewed in cross-sections. The periodicity D as well as the banding pattern were normal.
通过电子显微镜研究了以1.5和6毫当量/千克体重的剂量用氯化锂处理后小鼠皮肤胶原纤维的结构。在连续30天的实验期结束后1天、1个月、2个月和6个月处死动物。使用临床常用的第一剂量时，尽管存在胶原纤维正常的区域，但也有一些区域的纤维特征性平行排列完全丧失或仅部分区域保留。使用高剂量时，大多数区域的纤维正常排列被无规则排列所取代。在这两种情况下，胶原纤维的平均直径显著减小，并且与对照组的胶原纤维相比，宽度和形状的变异性更高。此外，还发现许多异常纤维簇，其直径可达260纳米（对照为109纳米），横截面观察时轮廓高度不规则。周期D以及条纹图案均正常。
