Tzaphlidou M, Kounadi E, Leontiou I, Matthopoulos D P, Glaros D
Laboratory of Medical Physics, Medical School, University of Ioannina, Greece.
Int J Radiat Biol. 1997 Jan;71(1):109-15. doi: 10.1080/095530097144481.
The structure of mouse skin collagen fibrils after treatment with 0.5, 1 and 2.5 Gy gamma-irradiation was studied by electron microscopy. Animals were sacrificed 1, 4 and 8 weeks after irradiation. Although there were areas where the normal parallel packing of fibrils was retained in some regions packing was interrupted by abnormal fibrils and in some cases helical twisting was apparent. Irradiated collagen fibrils had a lower mean diameter compared with normal and a large variability in width. The diameter of 0.5 Gy irradiated fibrils returned to normal by 4 or 8 weeks after irradiation. Clusters of abnormal fibrils were found when viewed in cross-sections. Their number and size was reversibly dependent on the dose level. All fibrils retained normal banding periodicity. Computer analysis of irradiated and control patterns led to the conclusion that 0.5-2.5 Gy gamma-irradiation had no considerable effect in modifying the charge distribution along the mouse skin collagen fibril.
通过电子显微镜研究了用0.5、1和2.5 Gy伽马射线辐照后小鼠皮肤胶原纤维的结构。在辐照后1、4和8周处死动物。尽管在某些区域仍保留了原纤维正常的平行排列，但在一些区域，排列被异常原纤维打断，在某些情况下还明显出现螺旋扭曲。与正常胶原纤维相比，辐照后的胶原纤维平均直径较小，宽度变化较大。0.5 Gy辐照的原纤维直径在辐照后4周或8周恢复正常。在横切面上观察到异常原纤维簇。它们的数量和大小与剂量水平呈可逆相关。所有原纤维均保留正常的条纹周期。对辐照和对照模式进行计算机分析得出结论，0.5 - 2.5 Gy伽马射线辐照对改变小鼠皮肤胶原纤维上的电荷分布没有显著影响。
