De Mot R, Nagy I, Schoofs G, Vanderleyden J
F.A. Janssens Laboratory of Genetics, Catholic University of Leuven, Heverlee, Belgium.
Can J Microbiol. 1994 May;40(5):403-7. doi: 10.1139/m94-066.
Sequence analysis of a 5173-bp genomic fragment from the nocardioform actinomycete Rhodococcus sp. strain NI86/21 revealed the presence of two genes, eutB and eutC, encoding the putative homologues of the large and small subunits of the ethanolamine ammonia-lyase, respectively, from Salmonella typhimurium. This is the first report of the characterization of these genes in a Gram-positive species. Immediately upstream of eutB, a gene encoding a putative permease of the APC (amino acids, polyamines, choline) transporter family was located. At present, no other Gram-positive members of this permease family are known. The translational coupling of these eut genes suggests an operon-like organization of the ethanolamine genes in Rhodococcus species. A truncated open reading frame downstream of eutC contained an N-terminal motif characteristic of membrane-anchored lipoproteins.
对诺卡氏放线菌红球菌属菌株NI86/21的一段5173bp基因组片段进行序列分析，结果显示存在两个基因，即eutB和eutC，它们分别编码来自鼠伤寒沙门氏菌的乙醇胺氨裂解酶大亚基和小亚基的假定同源物。这是首次在革兰氏阳性菌中对这些基因进行表征的报告。在eutB的紧邻上游，定位了一个编码APC（氨基酸、多胺、胆碱）转运蛋白家族假定通透酶的基因。目前，该通透酶家族尚无其他革兰氏阳性成员为人所知。这些eut基因的翻译偶联表明红球菌属中乙醇胺基因呈操纵子样组织形式。eutC下游的一个截短开放阅读框含有膜锚定脂蛋白特有的N端基序。
