De Mot R, Nagy I, Schoofs G, Vanderleyden J
F.A. Janssens Laboratory of Genetics, Catholic University of Leuven, Heverlee, Belgium.
Gene. 1994 May 27;143(1):91-3. doi: 10.1016/0378-1119(94)90610-6.
Sequence analysis of a 5753-bp genomic fragment of Rhodococcus sp. NI86/21 revealed the presence of three homologues of known cobalamin biosynthetic genes. One of these genes encodes a protein showing strong homology with the precorrin-6x reductase (CobK) of Pseudomonas denitrificans. In addition, the rhodococcal homologue of the corrin methyltransferases, CobM from P. denitrificans and CbiF from Salmonella typhimurium, was identified. The protein deduced from a third Rhodococcus gene aligns well with CobL of P. denitrificans and with the protein pair CbiE/CbiT of S. typhimurium, which are involved in corrin methylation and decarboxylation.
