Gordon A S, Howell L D, Harwood V
Department of Biological Sciences, Old Dominion University, Norfolk, VA 23529.
Can J Microbiol. 1994 May;40(5):408-11. doi: 10.1139/m94-067.
The influence of copper on the growth of Escherichia coli, Pseudomonas aeruginosa, Bacillus cereus, Bacillus subtilis, Vibrio parahaemolyticus, Vibrio alginolyticus (three strains), and an unidentified Vibrio sp. was examined in batch cultures. The effects of copper at micromolar concentrations varied from undetectable to complete growth inhibition. Each strain was able to recover from a growth lag observed after copper addition at a characteristic concentration. Copper concentrations that allowed recovery ranged from 25 to 150 microM. Extracellular proteins in the medium of cultures that had recovered from copper stress were compared with those from control cultures. Protein profiles were analyzed for the presence of proteins similar to extracellular copper-binding proteins (CuBP) previously reported in V. alginolyticus. CuBP-like proteins were found in each Vibrio sp. examined. A protein of similar molecular mass was also detected in copper-stressed cultures of P. aeruginosa and not in control cultures. Escherichia coli and Bacillus spp. did not produce CuBP-like proteins. The data show that CuBP-like proteins are not produced by all bacteria in response to copper stress and indicate that such proteins are common in marine Vibrio spp.
在分批培养中研究了铜对大肠杆菌、铜绿假单胞菌、蜡样芽孢杆菌、枯草芽孢杆菌、副溶血性弧菌、溶藻弧菌（三株）以及一株未鉴定的弧菌生长的影响。微摩尔浓度的铜的影响从无法检测到完全生长抑制各不相同。每种菌株都能够从在特征浓度添加铜后观察到的生长滞后期恢复。允许恢复的铜浓度范围为25至150微摩尔。将从铜胁迫中恢复的培养物培养基中的细胞外蛋白质与对照培养物中的进行比较。分析蛋白质谱以寻找与先前在溶藻弧菌中报道的细胞外铜结合蛋白（CuBP）相似的蛋白质的存在。在所检查的每种弧菌中都发现了类似CuBP的蛋白质。在铜胁迫的铜绿假单胞菌培养物中也检测到了分子量相似的一种蛋白质，而在对照培养物中未检测到。大肠杆菌和芽孢杆菌属不产生类似CuBP的蛋白质。数据表明，并非所有细菌都会因铜胁迫而产生类似CuBP的蛋白质，并表明此类蛋白质在海洋弧菌属中很常见。
