Sarkozi E, Askanas V, Johnson S A, McFerrin J, Engel W K
USC Neuromuscular Center, University of Southern California School of Medicine, Los Angeles 90017.
Exp Neurol. 1994 Jul;128(1):27-33. doi: 10.1006/exnr.1994.1109.
Regenerating muscle fibers in 25 human muscle biopsies, obtained from patients with a variety of neuromuscular diseases, manifested increased mRNA for the beta-amyloid precursor protein (beta APP) that contains the Kunitz-type protease inhibitor (KPI) motif, whereas adult human muscle fibers were negative in their vast non-extrajunctional region. Aneurally cultured normal human muscle fibers also expressed strong KPI-beta APP mRNA signal, which became significantly down-regulated during muscle differentiation. Our study demonstrates that in human muscle KPI-beta APP mRNA is developmentally regulated, and it suggests that beta APP may play a role in human muscle development.
从患有多种神经肌肉疾病的患者身上获取的25份人类肌肉活检样本中，再生的肌纤维显示出包含Kunitz型蛋白酶抑制剂（KPI）基序的β-淀粉样前体蛋白（βAPP）的mRNA增加，而成人人类肌纤维在其广阔的非神经肌接头区域呈阴性。无神经培养的正常人肌纤维也表达强烈的KPI-βAPP mRNA信号，该信号在肌肉分化过程中显著下调。我们的研究表明，在人类肌肉中KPI-βAPP mRNA受发育调控，这表明βAPP可能在人类肌肉发育中起作用。
